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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB7163 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB7163, RRID:AB_1577068
- Product name
- MBD2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of MBD2.
- Antigen sequence
C-RNDPLNQNKGKPDLN- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Specific binding of the methyl binding domain protein 2 at the BRCA1-NBR2 locus.
Auriol E, Billard LM, Magdinier F, Dante R
Nucleic acids research 2005;33(13):4243-54
Nucleic acids research 2005;33(13):4243-54
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- MBD2 polyclonal antibody (Cat # PAB7163) (0.3 ug/mL) staining of Jurkat cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- MBD2 polyclonal antibody (Cat # PAB7163) (2.5 ug/mL) staining of paraffin embedded human colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
