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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NB100-55415 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-55415, RRID:AB_838557
- Product name
- Goat Polyclonal MBD2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to resognize isoform 1 (NP_003918.1) only.
- Reactivity
- Human
- Host
- Goat
- Antigen sequence
RNDPLNQNKGKPDLN- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Control of the DNA methylation system component MBD2 by protein arginine methylation.
Specific binding of the methyl binding domain protein 2 at the BRCA1-NBR2 locus.
Tan CP, Nakielny S
Molecular and cellular biology 2006 Oct;26(19):7224-35
Molecular and cellular biology 2006 Oct;26(19):7224-35
Specific binding of the methyl binding domain protein 2 at the BRCA1-NBR2 locus.
Auriol E, Billard LM, Magdinier F, Dante R
Nucleic acids research 2005;33(13):4243-54
Nucleic acids research 2005;33(13):4243-54
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MBD2 Antibody [NB100-55415] - (0.3ug/ml) staining of Jurkat cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MBD2 Antibody [NB100-55415] - (2.5ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
