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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AP14193PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP14193PU-N, RRID:AB_1769549
- Product name
- anti MAPK15 (N-term)
- Antibody type
- Polyclonal
- Antigen
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human ERK8.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- (LEFT) Western blot analysis of ERK8 (arrow) using rabbit polyclonal ERK8 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAPK15 gene (Lane 2) (Origene Technologies). (RIGHT) Western blot analysis of anti-ERK8 Antibody (N-term) in T47D cell line lysates (35ug/lane). ERK8 (arrow) was detected using the purified Pab (1:60 dilution).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with ERK8 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
