Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB2954 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2954, RRID:AB_1577023
- Product name
- MAPK15 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of MAPK15.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references ERK8 down-regulates transactivation of the glucocorticoid receptor through Hic-5.
Characterization of the reversible phosphorylation and activation of ERK8.
Activation of the Erk8 mitogen-activated protein (MAP) kinase by RET/PTC3, a constitutively active form of the RET proto-oncogene.
Saelzler MP, Spackman CC, Liu Y, Martinez LC, Harris JP, Abe MK
The Journal of biological chemistry 2006 Jun 16;281(24):16821-32
The Journal of biological chemistry 2006 Jun 16;281(24):16821-32
Characterization of the reversible phosphorylation and activation of ERK8.
Klevernic IV, Stafford MJ, Morrice N, Peggie M, Morton S, Cohen P
The Biochemical journal 2006 Feb 15;394(Pt 1):365-73
The Biochemical journal 2006 Feb 15;394(Pt 1):365-73
Activation of the Erk8 mitogen-activated protein (MAP) kinase by RET/PTC3, a constitutively active form of the RET proto-oncogene.
Iavarone C, Acunzo M, Carlomagno F, Catania A, Melillo RM, Carlomagno SM, Santoro M, Chiariello M
The Journal of biological chemistry 2006 Apr 14;281(15):10567-76
The Journal of biological chemistry 2006 Apr 14;281(15):10567-76
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of MAPK15 (arrow) using rabbit MAPK15 polyclonal antibody (Cat # PAB2954). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAPK15 gene (Lane 2) (Origene Technologies).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of MAPK15 polyclonal antibody (Cat # PAB2954) in T-47D cell line lysates (35 ug/lane). MAPK15 (arrow) was detected using the purified polyclonal antibody.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MAPK15 polyclonal antibody (Cat # PAB2954) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)