Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-28587 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MAPK15 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLaS3. Predicted reactivity: Human (93%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using MAPK15 Polyclonal Antibody (Product # PA5-28587). Sample. A: 30 µg of HeLa S3 whole cell lysate. 7.5% SDS PAGE. MAPK15 Polyclonal Antibody (Product # PA5-28587) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MAPK15/ERK7 in methanol-fixed A431 cells using a MAPK15/ERK7 polyclonal antibody (Product # PA5-28587) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MAPK15 Polyclonal Antibody detects MAPK15 protein at cytosol on human lung by immunohistochemical analysis. Sample: Paraffin-embedded lung. MAPK15 Polyclonal Antibody (Product # PA5-28587) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.