FNab08866

antibody from FineTest Biotech

Targeting: TOP1MT

Western blot

ELISA

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Validation data

Product number: FNab08866 - Provider product page
Provider: FineTest Biotech
Product name: TOP1MT antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone(By similarity).
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Antigen sequence: topoisomerase(DNA) I, mitochondrial
Epitope: IgG
Isotype: IgG
Vial size: 100¦Ìg
Concentration: Immunogen affinity purified
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20¡æ for 12 months(Avoid repeated freeze / thaw cycles.)
Handling: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20¡æ for 12 months(Avoid repeated freeze / thaw cycles.)

TOP1MT protein structure - FNab08866 shown in red.