LS-C775079

antibody from LSBio

Targeting: TCF4 bHLHb19, E2-2, ITF2, SEF2-1B

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C775079

Provider

LSBio

Product name

TCF4 Antibody (Atto 700) LS-C775079

Antibody type

Polyclonal

Description

Peptide Affinity Purified

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Near infrared dye

Storage

Store at -20°C.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western blot analysis of Mouse liver lysate showing detection of ~71.3 kDa TCF4 protein using Rabbit Anti-TCF4 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse liver lysate. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-TCF4 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~71.3 kDa. Other Band(s): ~45 kDa isoform.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry analysis using Rabbit Anti-TCF4 Polyclonal Antibody. Tissue: Kidney. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-TCF4 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.