PA5-47791

antibody from Invitrogen Antibodies

Targeting: SEMA5A SEMAF, semF

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-47791

Provider

Invitrogen Antibodies

Product name

SEMA5A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human, Mouse, Rat

Host

Sheep

Isotype

IgG

Vial size

100 µg

Concentration

0.2 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis from lysates of ßTC-6 mouse beta cell insulinoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-human/mouse/Rat Semaphorin 5A Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47791) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for Semaphorin 5A at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SEMA5A in βTC‚6 mouse beta cell insulinoma cell line. Samples were incubated in SEMA5A polyclonal antibody (Product # PA5-47791) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Sheep IgG secondary antibody. A specific band was detected for Semaphorin 5A at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of SEMA5A in immersion fixed βTC‚6 mouse beta cell insulinoma cell line. Samples were incubated in SEMA5A polyclonal antibody (Product # PA5-47791) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of SEMA5A in immersion fixed paraffin-embedded sections of human pancreas. Samples were incubated in SEMA5A polyclonal antibody (Product # PA5-47791) using a dilution of 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hemotoxylin (blue). Specific staining was localized to the plasma membranes of epithelial cells.