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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Other assay [2]
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- Product number
- PA5-64182 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LRP2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: NNDRIYWSDF KEDVIETIKY DGTDRRVIAK EAMNPYSLDI FEDQLYWISK EKGEVWKQNK FGQGKKEKTL VVNPWLTQVR IFHQLRYNKS VP
- Concentration
- 0.1 mg/mL
Submitted references Complete remission of nephrotic syndrome in a young woman with anti-LRP2 nephropathy after immunosuppressive therapy.
Zhu X, Tu L, Liu S, You H, Xue J, Hao C
BMC nephrology 2020 Aug 24;21(1):364
BMC nephrology 2020 Aug 24;21(1):364
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot was performed using Anti-LRP2 Polyclonal Antibody (Product # PA5-64182) and a 522 kDa band corresponding to LRP2 was observed across in Mouse Kidney and Rat Kidney which are reported to be the highly expressed tissue models. Tissue extracts (30 µg lysate) of Mouse Skeletal Muscle (Lane 1), Rat Skeletal Muscle (Lane 2), Mouse Liver (Lane 3), Rat Liver (Lane 4), Mouse Lung (Lane 5), Rat Lung (Lane 6), Mouse Kidney (Lane 7) and Rat Kidney (Lane 8) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of LRP2 in human cell line HEK 293 shows positivity in mitochondria. Samples were probed using a LRP2 Polyclonal Antibody (Product # PA5-64182).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of LRP2 in human kidney tissue shows strong membranous positivity in cells in tubules. Samples were probed using a LRP2 Polyclonal Antibody (Product # PA5-64182).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of LRP2 in human kidney and lymph node tissues using LRP2 Polyclonal Antibody (Product # PA5-64182). Corresponding LRP2 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of LRP2 in human lymph node using LRP2 Polyclonal Antibody (Product # PA5-64182) shows low expression as expected.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 2 Colocalization of LRP2 and IgG on the patient's renal biopsy sample. a - c Immunofluorescence experiments showed positive staining along the apical membrane of the proximal tubules for: a LRP2 using a rabbit polyclonal antibody and b IgG. c Strong colocalization of LRP2 and IgG along the apical membrane of the proximal tubules
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4 Immunofluorescence performed on cryosections of normal human kidney tissue. a Staining for LRP2 using a rabbit antibody along the apical membrane of the proximal tubular epithelium. b Indirect immunofluorescence of serum from a patient with anti-LRP2 nephropathy on a normal human kidney. c Strong colocalization of LRP2 and serum antibodies
