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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- ELISA [1]
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- Product number
- H00003693-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00003693-M01, RRID:AB_489768
- Product name
- ITGB5 monoclonal antibody (M01), clone 2C4
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant ITGB5.
- Antigen sequence
TASFEVSLEARSCPSRHTEHVFALRPVGFRDSLEV
GVTYNCTCGCSVGLEPNSARCNGSGTYVCGLCECS
PGYLGTRCECQDGENQSVYQNLCREA- Isotype
- IgG
- Antibody clone number
- 2C4
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references β5 integrin is the major contributor to the αVintegrin-mediated blockade of HIV-1 replication.
Ballana E, Pauls E, Clotet B, Perron-Sierra F, Tucker GC, Esté JA
Journal of immunology (Baltimore, Md. : 1950) 2011 Jan 1;186(1):464-70
Journal of immunology (Baltimore, Md. : 1950) 2011 Jan 1;186(1):464-70
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ITGB5 monoclonal antibody (M01), clone 2C4 Western Blot analysis of ITGB5 expression in HeLa ( Cat # L013V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of ITGB5 expression in transfected 293T cell line by ITGB5 monoclonal antibody (M01), clone 2C4.Lane 1: ITGB5 transfected lysate(88.1 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged ITGB5 is approximately 0.3ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
