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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [3]
- ELISA [1]
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- Product number
- H00003693-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00003693-M01, RRID:AB_539888
- Product name
- Mouse Monoclonal Integrin beta 5 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. ITGB5 - integrin, beta 5
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Combinatorial fibronectin and laminin signaling promote highly efficient cardiac differentiation of human embryonic stem cells.
β5 integrin is the major contributor to the αVintegrin-mediated blockade of HIV-1 replication.
Sa S, Wong L, McCloskey KE
BioResearch open access 2014 Aug 1;3(4):150-61
BioResearch open access 2014 Aug 1;3(4):150-61
β5 integrin is the major contributor to the αVintegrin-mediated blockade of HIV-1 replication.
Ballana E, Pauls E, Clotet B, Perron-Sierra F, Tucker GC, Esté JA
Journal of immunology (Baltimore, Md. : 1950) 2011 Jan 1;186(1):464-70
Journal of immunology (Baltimore, Md. : 1950) 2011 Jan 1;186(1):464-70
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Integrin beta 5 Antibody (2C4) [H00003693-M01] - Analysis of ITGB5 over-expressed 293 cell line, cotransfected with ITGB5 Validated Chimera RNAi ( Cat # H00003693-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ITGB5 monoclonal antibody (M01), clone 2C4 (Cat # H00003693-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Integrin beta 5 Antibody (2C4) [H00003693-M01] - Analysis of ITGB5 expression in transfected 293T cell line by ITGB5 monoclonal antibody (M01), clone 2C4.Lane 1: ITGB5 transfected lysate(88.1 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Integrin beta 5 Antibody (2C4) [H00003693-M01] - ITGB5 monoclonal antibody (M01), clone 2C4 Analysis of ITGB5 expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Integrin beta 5 Antibody (2C4) [H00003693-M01] - Detection limit for recombinant GST tagged ITGB5 is approximately 0.3ng/ml as a capture antibody.
