720284

antibody from Invitrogen Antibodies

Targeting: DRD5 DRD1B, DRD1L2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

720284

Provider

Invitrogen Antibodies

Product name

DRD5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

These Polyclonal antibodies are of rabbit origin developed by immunizing animals with proteins or peptides. The polyclonal antibody is purified by affinity purification from the rabbit sera generated after immunizing the rabbits with a specific type of protein or peptide. The purified antibody is tested for its functionality in various relevant research applications. The antibody is developed for Research Use Only and is non-hazardous or non-infectious in nature.

Concentration

0.5 mg/mL

References

Submitted references

Biochemical Neuroadaptations in the Rat Striatal Dopaminergic System after Prolonged Exposure to Methamphetamine Self-Administration.

Jayanthi S, Ladenheim B, Sullivan P, McCoy MT, Krasnova IN, Goldstein DS, Cadet JL
International journal of molecular sciences 2022 Sep 3;23(17)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on membrane enriched cell extracts (30 µg lysate) of Neuro-2a (Lane 1) and tissue extracts of Rat Brain (Lane 2) and Mouse Brain (Lane 3). The blots were probed with Dopamine Receptors- D5 Rabbit Polyclonal antibody (Product # 720284, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A ~ 35 kDa band corresponding to Dopamine Receptors- D5 was observed. Additionally ~50 kDa band was observed in Neuro-2a cells. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10% Bis-Tris gel (Product # NP0301BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot®2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on membrane enriched cell extracts (30 µg lysate) of Neuro-2a (Lane 1) and tissue extracts of Rat Brain (Lane 2) and Mouse Brain (Lane 3). The blots were probed with Dopamine Receptors- D5 Rabbit Polyclonal antibody (Product # 720284, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A ~ 35 kDa band corresponding to Dopamine Receptors- D5 was observed. Additionally ~50 kDa band was observed in Neuro-2a cells. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10% Bis-Tris gel (Product # NP0301BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot®2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).