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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- ELISA [1]
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- Product number
- H00008780-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00008780-M02, RRID:AB_2178108
- Product name
- Mouse Monoclonal RIOK3 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. RIOK3 - RIO kinase 3 (yeast)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RIOK3 Antibody (3G11) [H00008780-M02] - Analysis of RIOK3 over-expressed 293 cell line, cotransfected with RIOK3 Validated Chimera RNAi ( Cat # H00008780-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RIOK3 monoclonal antibody (M02) clone 3G11 (Cat # H00008780-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RIOK3 Antibody (3G11) [H00008780-M02] - Analysis of RIOK3 expression in transfected 293T cell line by RIOK3 monoclonal antibody (M02), clone 3G11.Lane 1: RIOK3 transfected lysate(59.1 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RIOK3 Antibody (3G11) [H00008780-M02] - RIOK3 monoclonal antibody (M02), clone 3G11 Analysis of RIOK3 expression in K-562.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: RIOK3 Antibody (3G11) [H00008780-M02] - Detection limit for recombinant GST tagged RIOK3 is approximately 0.03ng/ml as a capture antibody.
