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- References [3]
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- Western blot [3]
- ELISA [1]
- Immunoprecipitation [1]
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- Product number
- H00008780-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00008780-M01, RRID:AB_547722
- Product name
- Mouse Monoclonal RIOK3 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. RIOK3 - RIO kinase 3 (yeast)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references The atypical protein kinase RIOK3 contributes to glioma cell proliferation/survival, migration/invasion and the AKT/mTOR signaling pathway.
Hypoxic regulation of RIOK3 is a major mechanism for cancer cell invasion and metastasis.
Genomic alterations link Rho family of GTPases to the highly invasive phenotype of pancreas cancer.
Zhang T, Ji D, Wang P, Liang D, Jin L, Shi H, Liu X, Meng Q, Yu R, Gao S
Cancer letters 2018 Feb 28;415:151-163
Cancer letters 2018 Feb 28;415:151-163
Hypoxic regulation of RIOK3 is a major mechanism for cancer cell invasion and metastasis.
Singleton DC, Rouhi P, Zois CE, Haider S, Li JL, Kessler BM, Cao Y, Harris AL
Oncogene 2015 Sep 3;34(36):4713-22
Oncogene 2015 Sep 3;34(36):4713-22
Genomic alterations link Rho family of GTPases to the highly invasive phenotype of pancreas cancer.
Kimmelman AC, Hezel AF, Aguirre AJ, Zheng H, Paik JH, Ying H, Chu GC, Zhang JX, Sahin E, Yeo G, Ponugoti A, Nabioullin R, Deroo S, Yang S, Wang X, McGrath JP, Protopopova M, Ivanova E, Zhang J, Feng B, Tsao MS, Redston M, Protopopov A, Xiao Y, Futreal PA, Hahn WC, Klimstra DS, Chin L, DePinho RA
Proceedings of the National Academy of Sciences of the United States of America 2008 Dec 9;105(49):19372-7
Proceedings of the National Academy of Sciences of the United States of America 2008 Dec 9;105(49):19372-7
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RIOK3 Antibody (4A12-1D2) [H00008780-M01] - Western Blot analysis of RIOK3 expression in Hela NE ( Cat # L013V3 ).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RIOK3 Antibody (4A12-1D2) [H00008780-M01] - Analysis of RIOK3 expression in transfected 293T cell line by RIOK3 monoclonal antibody (M01), clone 4A12-1D2.Lane 1: RIOK3 transfected lysate(59.1 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RIOK3 Antibody (4A12-1D2) [H00008780-M01] - RIOK3 monoclonal antibody (M01), clone 4A12-1D2. Analysis of RIOK3 expression in MCF-7.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: RIOK3 Antibody (4A12-1D2) [H00008780-M01] - Detection limit for recombinant GST tagged RIOK3 is approximately 0.3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: RIOK3 Antibody (4A12-1D2) [H00008780-M01] - Analysis of RIOK3 transfected lysate using anti-RIOK3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RIOK3 MaxPab rabbit polyclonal antibody.
