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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- 13593-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RIOK3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: RRSARLHEK KEHSTAEKAV DPKTRLLMYK MVNSGMLETI TGCISTGKES VVFHAYGGSM EDEKEDSKVI PTECAIKVFK TTLNEFKNRD KYIKDDFRFK DRFSKLNPRK IIRMWAEKEM HNLARMQRAG IPCPTVVLLK KHILVMSFIG HDQVPAPKLK EVKLNSEEMK EAYYQTLHLM RQLYHECTLV HADLSEYNML WHAGKVWLID VSQSVEPTHP HGLEFLFRDC RNVSQKGGVK EALSERELFN AVSGLNITAD NEADFLAEIE ALEKMNEDHV QKNGRKAASF LKDDGDPPLL YDE (215-516 aa encoded by BC039729)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.15 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse testis tissue were subjected to SDS PAGE followed by western blot with 13593-1-AP (RIOK3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF7 cells were subjected to SDS PAGE followed by western blot with 13593-1-AP (RIOK3 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MCF-7 cells using 13593-1-AP (RIOK3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 13593-1-AP (RIOK3 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 13593-1-AP (RIOK3 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-RIOK3 (IP:13593-1-AP, 4ug; Detection:13593-1-AP 1:600) with mouse testis tissue lysate 4000ug.
