200-401-909

antibody from Rockland Immunochemicals, Inc.

Targeting: RREB1 HNT

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

200-401-909

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#200-401-909, RRID:AB_2253857

Product name

Anti-RREB1 (RABBIT) Antibody - 200-401-909

Antibody type

Polyclonal

Vial size

500 µl

References

Submitted references

Role of the PLDLS-binding cleft region of CtBP1 in recruitment of core and auxiliary components of the corepressor complex.

Kuppuswamy M, Vijayalingam S, Zhao LJ, Zhou Y, Subramanian T, Ryerse J, Chinnadurai G
Molecular and cellular biology 2008 Jan;28(1):269-81

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blot using Rockland's Protein A Purified anti-RREB1 antibody shows detection of a predominant band believed to be RREB1 in various cell lysates (1 - HEK293, 2 - RFP-RREB transfected HEK293, 3 - M460 and 4 - T1165). All lysates were loaded at 20 ?g per lane and separated by SDS-PAGE. After transfer to nitrocellulose, the membrane was probed with the primary antibody diluted to 1:1,000. The membrane was washed and reacted with IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (611-132-122). IRDye800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Size estimation was made by comparison to prestained MW markers as indicated. Personal Communication, Shuling Zhang, CCR, NCI, Bethesda, MD.

Validation comment

Western Blot