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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
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- Product number
- PAB10322 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10322, RRID:AB_1713267
- Product name
- Rreb1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against recombinant Rreb1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references p16 INK4a gene promoter variation and differential binding of a repressor, the ras-responsive zinc-finger transcription factor, RREB.
Cdkn2a, the cyclin-dependent kinase inhibitor encoding p16INK4a and p19ARF, is a candidate for the plasmacytoma susceptibility locus, Pctr1.
Zhang S, Qian X, Redman C, Bliskovski V, Ramsay ES, Lowy DR, Mock BA
Oncogene 2003 Apr 17;22(15):2285-95
Oncogene 2003 Apr 17;22(15):2285-95
Cdkn2a, the cyclin-dependent kinase inhibitor encoding p16INK4a and p19ARF, is a candidate for the plasmacytoma susceptibility locus, Pctr1.
Zhang S, Ramsay ES, Mock BA
Proceedings of the National Academy of Sciences of the United States of America 1998 Mar 3;95(5):2429-34
Proceedings of the National Academy of Sciences of the United States of America 1998 Mar 3;95(5):2429-34
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- Abnova Corporation (provider)
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- Experimental details
- Western blot using Rreb1 polyclonal antibody (Cat # PAB10322) shows detection ofa predominant band believed to be Rreb1 invarious cell lysates (1 - HEK293, 2 - RFP-Rreb transfected HEK293, 3 - M460 and 4 - T1165).All lysates were loaded at 20 ug per lane and separated by SDS-PAGE.After transfer to nitrocellulose, the membrane was probed with the primary antibody diluted to 1:1,000.The membrane was washed and reacted with IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX.IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.IRDye is a trademark of LI-COR, Inc. Size estimation was made by comparison to prestained MW markers as indicated.Personal Communication, Shuling Zhang, CCR, NCI, Bethesda, MD.
