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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- STJ91087 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-Phospho-CDKN1C-Thr310 antibody (250-330) (STJ91087)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody anti-Phospho-Cyclin-Dependent Kinase Inhibitor 1c-Thr310 (250-330) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry and ELISA research applications.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
NA- Epitope
- NA
- Isotype
- IgG
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
- Handling
- NA
No comments: Submit comment
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Western blot analysis of lysates from K562 cells treated with insulin 0.01U/ml 15', using p57 Kip2 (Phospho-Thr310) Antibody. The lane on the right is blocked with the phospho peptide.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using p57 Kip2 (Phospho-Thr310) Antibody
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HUVEC cells treated with serum 20% 30', using p57 Kip2 (Phospho-Thr310) Antibody. The picture on the right is blocked with the phospho peptide.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of paraffin-embedded human placenta, using p57 Kip2 (Phospho-Thr310) Antibody. The picture on the right is blocked with the phospho peptide.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
