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LearnA02152
antibody from Boster Biological Technology
Targeting: TNFRSF10A
Apo2, CD261, DR4, TRAILR-1, TRAILR1
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- A02152 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-DR4/TNFRSF10A Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for DR4/TNFRSF10A detection. Host: Rabbit.Size: 100μg/vial. Tested applications: Flow Cytometry. Reactive species: Human. DR4/TNFRSF10A information: Molecular Weight: 50089 MW; Subcellular Localization: Membrane; Single-pass type I membrane protein; Tissue Specificity: Widely expressed. High levels are found in spleen, peripheral blood leukocytes, small intestine and thymus, but also in K-562 erythroleukemia cells, MCF-7 breast carcinoma cells and activated T-cells.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of DR4 using anti-DR4 antibody (A02152). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: rat spleen tissue lysates, lane 2: mouse spleen tissue lysates, lane 3: MCF-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DR4 antigen affinity purified polyclonal antibody (Catalog # A02152) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DR4 at approximately 50KD. The expected band size for DR4 is at 50KD.
- Additional image
