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LearnALX-804-297F-T100
antibody from Enzo Life Sciences
Targeting: TNFRSF10A
Apo2, CD261, DR4, TRAILR-1, TRAILR1
Immunocytochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [13]
- Comments [0]
- Validations
- Immunocytochemistry [3]
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- Product number
- ALX-804-297F-T100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-804-297F-T100, RRID:AB_11178260
- Product name
- TRAIL-R1 (human) monoclonal antibody (HS101) (FITC conjugate)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full length protein
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- HS101
- Vial size
- 100 tests
- Storage
- +4°C
- Handling
- Do not freeze. Protect from light.
Submitted references JNK inhibition sensitises hepatocellular carcinoma cells but not normal hepatocytes to the TNF-related apoptosis-inducing ligand.
Resistance to FasL and tumor necrosis factor-related apoptosis-inducing ligand-mediated apoptosis in Sezary syndrome T-cells associated with impaired death receptor and FLICE-inhibitory protein expression.
P60-c-src suppresses apoptosis through inhibition of caspase 8 activation in hepatoma cells, but not in primary hepatocytes.
Casein kinase I attenuates tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis by regulating the recruitment of fas-associated death domain and procaspase-8 to the death-inducing signaling complex.
IFNalpha-stimulated neutrophils and monocytes release a soluble form of TNF-related apoptosis-inducing ligand (TRAIL/Apo-2 ligand) displaying apoptotic activity on leukemic cells.
Enhanced caspase-8 recruitment to and activation at the DISC is critical for sensitisation of human hepatocellular carcinoma cells to TRAIL-induced apoptosis by chemotherapeutic drugs.
TRAIL regulates normal erythroid maturation through an ERK-dependent pathway.
cFLIPL inhibits tumor necrosis factor-related apoptosis-inducing ligand-mediated NF-kappaB activation at the death-inducing signaling complex in human keratinocytes.
Silencing of death receptor and caspase-8 expression in small cell lung carcinoma cell lines and tumors by DNA methylation.
Proteasome inhibition results in TRAIL sensitization of primary keratinocytes by removing the resistance-mediating block of effector caspase maturation.
TNF-related apoptosis-inducing ligand mediates tumoricidal activity of human monocytes stimulated by Newcastle disease virus.
Caspase-10 is recruited to and activated at the native TRAIL and CD95 death-inducing signalling complexes in a FADD-dependent manner but can not functionally substitute caspase-8.
Mitogen-activated protein kinase/extracellular signal-regulated kinase signaling in activated T cells abrogates TRAIL-induced apoptosis upstream of the mitochondrial amplification loop and caspase-8.
Mucha SR, Rizzani A, Gerbes AL, Camaj P, Thasler WE, Bruns CJ, Eichhorst ST, Gallmeier E, Kolligs FT, Göke B, De Toni EN
Gut 2009 May;58(5):688-98
Gut 2009 May;58(5):688-98
Resistance to FasL and tumor necrosis factor-related apoptosis-inducing ligand-mediated apoptosis in Sezary syndrome T-cells associated with impaired death receptor and FLICE-inhibitory protein expression.
Contassot E, Kerl K, Roques S, Shane R, Gaide O, Dupuis M, Rook AH, French LE
Blood 2008 May 1;111(9):4780-7
Blood 2008 May 1;111(9):4780-7
P60-c-src suppresses apoptosis through inhibition of caspase 8 activation in hepatoma cells, but not in primary hepatocytes.
De Toni EN, Kuntzen C, Gerbes AL, Thasler WE, Sonuc N, Mucha SR, Camaj P, Bruns C, Göke B, Eichhorst ST
Journal of hepatology 2007 Apr;46(4):682-91
Journal of hepatology 2007 Apr;46(4):682-91
Casein kinase I attenuates tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis by regulating the recruitment of fas-associated death domain and procaspase-8 to the death-inducing signaling complex.
Izeradjene K, Douglas L, Delaney AB, Houghton JA
Cancer research 2004 Nov 1;64(21):8036-44
Cancer research 2004 Nov 1;64(21):8036-44
IFNalpha-stimulated neutrophils and monocytes release a soluble form of TNF-related apoptosis-inducing ligand (TRAIL/Apo-2 ligand) displaying apoptotic activity on leukemic cells.
Tecchio C, Huber V, Scapini P, Calzetti F, Margotto D, Todeschini G, Pilla L, Martinelli G, Pizzolo G, Rivoltini L, Cassatella MA
Blood 2004 May 15;103(10):3837-44
Blood 2004 May 15;103(10):3837-44
Enhanced caspase-8 recruitment to and activation at the DISC is critical for sensitisation of human hepatocellular carcinoma cells to TRAIL-induced apoptosis by chemotherapeutic drugs.
Ganten TM, Haas TL, Sykora J, Stahl H, Sprick MR, Fas SC, Krueger A, Weigand MA, Grosse-Wilde A, Stremmel W, Krammer PH, Walczak H
Cell death and differentiation 2004 Jul;11 Suppl 1:S86-96
Cell death and differentiation 2004 Jul;11 Suppl 1:S86-96
TRAIL regulates normal erythroid maturation through an ERK-dependent pathway.
Secchiero P, Melloni E, Heikinheimo M, Mannisto S, Di Pietro R, Iacone A, Zauli G
Blood 2004 Jan 15;103(2):517-22
Blood 2004 Jan 15;103(2):517-22
cFLIPL inhibits tumor necrosis factor-related apoptosis-inducing ligand-mediated NF-kappaB activation at the death-inducing signaling complex in human keratinocytes.
Wachter T, Sprick M, Hausmann D, Kerstan A, McPherson K, Stassi G, Bröcker EB, Walczak H, Leverkus M
The Journal of biological chemistry 2004 Dec 17;279(51):52824-34
The Journal of biological chemistry 2004 Dec 17;279(51):52824-34
Silencing of death receptor and caspase-8 expression in small cell lung carcinoma cell lines and tumors by DNA methylation.
Hopkins-Donaldson S, Ziegler A, Kurtz S, Bigosch C, Kandioler D, Ludwig C, Zangemeister-Wittke U, Stahel R
Cell death and differentiation 2003 Mar;10(3):356-64
Cell death and differentiation 2003 Mar;10(3):356-64
Proteasome inhibition results in TRAIL sensitization of primary keratinocytes by removing the resistance-mediating block of effector caspase maturation.
Leverkus M, Sprick MR, Wachter T, Mengling T, Baumann B, Serfling E, Bröcker EB, Goebeler M, Neumann M, Walczak H
Molecular and cellular biology 2003 Feb;23(3):777-90
Molecular and cellular biology 2003 Feb;23(3):777-90
TNF-related apoptosis-inducing ligand mediates tumoricidal activity of human monocytes stimulated by Newcastle disease virus.
Washburn B, Weigand MA, Grosse-Wilde A, Janke M, Stahl H, Rieser E, Sprick MR, Schirrmacher V, Walczak H
Journal of immunology (Baltimore, Md. : 1950) 2003 Feb 15;170(4):1814-21
Journal of immunology (Baltimore, Md. : 1950) 2003 Feb 15;170(4):1814-21
Caspase-10 is recruited to and activated at the native TRAIL and CD95 death-inducing signalling complexes in a FADD-dependent manner but can not functionally substitute caspase-8.
Sprick MR, Rieser E, Stahl H, Grosse-Wilde A, Weigand MA, Walczak H
The EMBO journal 2002 Sep 2;21(17):4520-30
The EMBO journal 2002 Sep 2;21(17):4520-30
Mitogen-activated protein kinase/extracellular signal-regulated kinase signaling in activated T cells abrogates TRAIL-induced apoptosis upstream of the mitochondrial amplification loop and caspase-8.
Söderström TS, Poukkula M, Holmström TH, Heiskanen KM, Eriksson JE
Journal of immunology (Baltimore, Md. : 1950) 2002 Sep 15;169(6):2851-60
Journal of immunology (Baltimore, Md. : 1950) 2002 Sep 15;169(6):2851-60
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Figure A: Strong signal intensity is obtained by FITC-conjugated MAb HS101 (Prod. No. ALX-804-297F) compared to FITC-conjugated control. Method: CV1/EBVA cells (1x10E6) transiently transfected with pCDNA3 encodingÊ TRAIL-R1, were incubated with FITC-conjugated MAb HS101 against TRAIL-R1 or FITC-conjugated matched isotype control (mouse IgG1). Antibodies were used at 5µg/ml.Technical Note: Signal intensity is comparable to a secondary (anti-mouse IgG1-FITC) antibody (data not shown).Figure B: Signal intensity achieved withÊ FITC-conjugated MAb HS101 compared toÊ additional biotin and streptavidin-PE fluorochrome amplification step. Method:ÊCV1/EBVA cells were either transiently transfected with pCDNA3 encodingÊ TRAIL-R1 (black histogram) or with empty expression vector pCDNA3 (white histogram). Cells (1x10E6) were first incubated with FITC-conjugated MAb HS101 (5µg/ml), followed by biotinylated anti-mouse IgG1 and streptavidin-PE.Technical Note: Signal increase is not significantly greater for biotin and streptavidin-PE amplification as opposed to FITC-conjugated MAb HS101 alone (Prod. No. ALX-804-297 for additional information).
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Receptor specifitiy of the FITC-conjugated MAb HS101 for TRAIL-R1 (Prod. No. ALX-804-297F). Method: CV1/EBNA cells were transfected either with the empty expression vector pCDNA3 (vector control) or pCDNA3 encoding TRAIL-R1 to TRAIL-R4. Two days after transfection 1x10E6 cells were stained with FITC-conjugated MAb HS101 against TRAIL-R1. All primary antibodies were used at 5µg/ml. Transfection efficiency was about 50% in all individual transfections.
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Flow cytometric detection of endogenous TRAIL-R1 surface expression on BJAB cells versus a TRAIL-R1 negative cell line, Jurkat. Method: BJAB or Jurkat cells (1x10E6) were stained with FITC-conjugated monoclonal antibody (MAb) HS101 against TRAIL-R1 (Prod. No. ALX-804-297F). Antibodies were used at 5µg/ml.
