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- Antigen structure
- References [8]
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- Western blot [2]
- Immunocytochemistry [2]
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- Product number
- H00004957-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00004957-M01, RRID:AB_1146453
- Product name
- Mouse Monoclonal ODF2 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. ODF2 - outer dense fiber of sperm tails 2 (1A1)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Quantitative analysis of sensitivity to a Wnt3a gradient in determination of the pole-to-pole axis of mitotic cells by using a microfluidic device.
Phosphatidylinositol phosphate kinase PIPKIγ and phosphatase INPP5E coordinate initiation of ciliogenesis.
Mutations in KIAA0586 Cause Lethal Ciliopathies Ranging from a Hydrolethalus Phenotype to Short-Rib Polydactyly Syndrome.
PIPKIγ targets to the centrosome and restrains centriole duplication.
Mutations of CEP83 cause infantile nephronophthisis and intellectual disability.
Transition fibre protein FBF1 is required for the ciliary entry of assembled intraflagellar transport complexes.
Centriole distal appendages promote membrane docking, leading to cilia initiation.
Evidence of asymmetric cell division and centrosome inheritance in human neuroblastoma cells.
Hiraiwa T, Nakai Y, Yamada TG, Tanimoto R, Kimura H, Matsumoto Y, Miki N, Hiroi N, Funahashi A
FEBS open bio 2018 Dec;8(12):1920-1935
FEBS open bio 2018 Dec;8(12):1920-1935
Phosphatidylinositol phosphate kinase PIPKIγ and phosphatase INPP5E coordinate initiation of ciliogenesis.
Xu Q, Zhang Y, Wei Q, Huang Y, Hu J, Ling K
Nature communications 2016 Feb 26;7:10777
Nature communications 2016 Feb 26;7:10777
Mutations in KIAA0586 Cause Lethal Ciliopathies Ranging from a Hydrolethalus Phenotype to Short-Rib Polydactyly Syndrome.
Alby C, Piquand K, Huber C, Megarbané A, Ichkou A, Legendre M, Pelluard F, Encha-Ravazi F, Abi-Tayeh G, Bessières B, El Chehadeh-Djebbar S, Laurent N, Faivre L, Sztriha L, Zombor M, Szabó H, Failler M, Garfa-Traore M, Bole C, Nitschké P, Nizon M, Elkhartoufi N, Clerget-Darpoux F, Munnich A, Lyonnet S, Vekemans M, Saunier S, Cormier-Daire V, Attié-Bitach T, Thomas S
American journal of human genetics 2015 Aug 6;97(2):311-8
American journal of human genetics 2015 Aug 6;97(2):311-8
PIPKIγ targets to the centrosome and restrains centriole duplication.
Xu Q, Zhang Y, Xiong X, Huang Y, Salisbury JL, Hu J, Ling K
Journal of cell science 2014 Mar 15;127(Pt 6):1293-305
Journal of cell science 2014 Mar 15;127(Pt 6):1293-305
Mutations of CEP83 cause infantile nephronophthisis and intellectual disability.
Failler M, Gee HY, Krug P, Joo K, Halbritter J, Belkacem L, Filhol E, Porath JD, Braun DA, Schueler M, Frigo A, Alibeu O, Masson C, Brochard K, Hurault de Ligny B, Novo R, Pietrement C, Kayserili H, Salomon R, Gubler MC, Otto EA, Antignac C, Kim J, Benmerah A, Hildebrandt F, Saunier S
American journal of human genetics 2014 Jun 5;94(6):905-14
American journal of human genetics 2014 Jun 5;94(6):905-14
Transition fibre protein FBF1 is required for the ciliary entry of assembled intraflagellar transport complexes.
Wei Q, Xu Q, Zhang Y, Li Y, Zhang Q, Hu Z, Harris PC, Torres VE, Ling K, Hu J
Nature communications 2013;4:2750
Nature communications 2013;4:2750
Centriole distal appendages promote membrane docking, leading to cilia initiation.
Tanos BE, Yang HJ, Soni R, Wang WJ, Macaluso FP, Asara JM, Tsou MF
Genes & development 2013 Jan 15;27(2):163-8
Genes & development 2013 Jan 15;27(2):163-8
Evidence of asymmetric cell division and centrosome inheritance in human neuroblastoma cells.
Izumi H, Kaneko Y
Proceedings of the National Academy of Sciences of the United States of America 2012 Oct 30;109(44):18048-53
Proceedings of the National Academy of Sciences of the United States of America 2012 Oct 30;109(44):18048-53
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ODF2 Antibody (1A1) [H00004957-M01] - (Transfected Lysate)
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ODF2 Antibody (1A1) [H00004957-M01] - Detection against Immunogen (36.63 KDa) .
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: ODF2 Antibody (1A1) [H00004957-M01] - Analysis of monoclonal antibody to ODF2 on HeLa cell . Antibody concentration 10 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: ODF2 Antibody (1A1) [H00004957-M01] - Co-staining of ODF2 (Cy3), DAPI and r-tubulin (Alexa Fluor 488) in RPE1 cell line. ODF2 stains the subdistal appendage of mother centriole. Cells were fixed with cold methanol, 10min. Blocked with 3% BSA for 30min at room temperature. ODF2 antibody was used at 1:100. Image from verified customer review.
