12058-1-AP

antibody from Invitrogen Antibodies

Targeting: ODF2 CT134, ODF84

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12058-1-AP

Provider

Invitrogen Antibodies

Product name

ODF2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: QQLKASVKN YEGMIDNYKS QVMKTRLEAD EVAAQLERCD KENKILKDEM NKEIEAARRQ FQSQLADLQQ LPDILKITEA KLAECQDQLQ GYERKNIDLT AIISDLRSRI EHQGDKLEMA REKHQASQKE NKQLSLKVDE LERKLEATSA QNIEFLQVIA KREEAIHQSQ LRLEEKTREC GTLARQLESA IEDARRQVEQ TKEHALSKER AAQNKILDLE TQLSRTKTEL SQLRRSRDDA DRRYQSRLQD LKDRLEQSES TNRSMQNYVQ FLKSSYANVF GDGPYSTFLT SSPIRSRSPP A (530-829 aa encoded by BC010629)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.53 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse testis tissue were subjected to SDS PAGE followed by western blot with 12058-1-AP (ODF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse lung tissue were subjected to SDS PAGE followed by western blot with 12058-1-AP (ODF2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HeLa cells using 12058-1-AP (ODF2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 12058-1-AP ( ODF2 antibody) at dilution of 1:100 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 12058-1-AP ( ODF2 antibody) at dilution of 1:100 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-ODF2 (IP:12058-1-AP, 5ug; Detection:12058-1-AP 1:500) with mouse testis tissue lysate 8000ug.