ABIN954754

antibody from antibodies-online

Targeting: SETD1B KIAA1076, KMT2G, Set1B

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

ABIN954754

Provider

antibodies-online

Product name

anti-SET Domain Containing 1B (SETD1B) (C-Term), (AA 685-720) antibody

Antibody type

Polyclonal

Antigen

KLH conjugated synthetic peptide between 685-720 amino acids from the C-terminal region of Human SET1B Genename: SETD1B

Description

Protein A column followed by peptide Affinity purification

Reactivity

Human, Mouse

Host

Rabbit

Epitope

C-Term,AA 685-720

Vial size

0.4 mL

Concentration

0.25 mg/mL

Storage

Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.

Handling

Avoid repeated freezing and thawing.

References

Submitted references

Wdr82 is a C-terminal domain-binding protein that recruits the Setd1A Histone H3-Lys4 methyltransferase complex to transcription start sites of transcribed human genes.

Lee JH, Skalnik DG
Molecular and cellular biology 2008 Jan;28(2):609-18

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Molecular regulation of H3K4 trimethylation by Wdr82, a component of human Set1/COMPASS.

Wu M, Wang PF, Lee JS, Martin-Brown S, Florens L, Washburn M, Shilatifard A
Molecular and cellular biology 2008 Dec;28(24):7337-44

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Identification and characterization of the human Set1B histone H3-Lys4 methyltransferase complex.

Lee JH, Tate CM, You JS, Skalnik DG
The Journal of biological chemistry 2007 May 4;282(18):13419-28

Western blot

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Western blot analysis using SET1B Antibody (C-term) (Cat. AP53868PU-N in Mouse heart tissue lysates (35ug/lane). This demonstrates the SET1B antibody detected the SET1B protein (arrow).