R20184

antibody from NSJ Bioreagents

Targeting: IGHA2

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

R20184

Provider

NSJ Bioreagents

Product name

Recombinant Human IgA2 Antibody

Antibody type

Recombinant

Description

Purified

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antibody clone number

RM125

Vial size

100 µg

Concentration

1 mg/ml in PBS with 50% Glycerol, 1% BSA and 0.09% sodium azide

Storage

Store the recombinant Human IgA2 antibody at -20°C.

ELISA

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Sandwich ELISA of human plasma using recombinant Human IgA2 antibody as the capture (100ng/well), and biotinylated anti-human light chains (κ+λ) antibody clone RM129 as the detect, followed by an alkaline phosphatase conjugated streptavidin.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Sandwich ELISA of human IgA2 using recombinant Human IgA2 antibody as the capture (100ng/well), and biotinylated anti-human light chains (κ+λ) antibody clone RM129 as the detect, followed by an alkaline phosphatase conjugated streptavidin.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

ELISA of human immunoglobulins shows recombinant Human IgA2 antibody reacts only to IgA2. No cross reactivity with IgA1, IgG, IgM, IgD, or IgE.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

ELISA Titration: the plate was coated with different amounts of human IgA2. A serial dilution of recombinant Human IgA2 antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human tonsil with recombinant Human IgA2 antibody (clone RM125). A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.