PA5-14350

antibody from Invitrogen Antibodies

Targeting: ADAMTS5 ADAMTS11, ADMP-2

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-14350

Provider

Invitrogen Antibodies

Product name

ADAMTS5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Hsa_circ_0134111 promotes intervertebral disc degeneration via sponging miR-578.

Yan P, Sun C, Luan L, Han J, Qu Y, Zhou C, Xu D
Cell death discovery 2022 Feb 8;8(1):55

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Bromodomain and Extra-terminal (BET) Protein Inhibitors Suppress Chondrocyte Differentiation and Restrain Bone Growth.

Niu N, Shao R, Yan G, Zou W
The Journal of biological chemistry 2016 Dec 23;291(52):26647-26657

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Matrix cross-linking-mediated mechanotransduction promotes posttraumatic osteoarthritis.

Kim JH, Lee G, Won Y, Lee M, Kwak JS, Chun CH, Chun JS
Proceedings of the National Academy of Sciences of the United States of America 2015 Jul 28;112(30):9424-9

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis using an ADAMTS5 polyclonal antibody (Product # PA5-14350) in mouse muscle tissue lysates (35 µg per lane).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 5 circ_0134111 overexpression induced proliferation, pro-inflammatory cytokine secretion, and ECM degradation in NP cells. A circ_0134111 overexpression promoted NP cell proliferation as shown by the CCK-8 assay. B Ectopic expression of circ_0134111 increased the cyclin D1 expression. C-F Overexpression of circ_0134111 induced C IL-6, D IL-8, E MMP-9, and F ADAMTS-5 expression as shown by qRT-PCR assay. G, H The expression of type II collagen and aggrecan was determined by qRT-PCR assay. Ectopic expression of circ_0134111 decreased G type II collagen and H aggrecan expression in NP cells. J The protein expression of MMP-9 and ADAMTS-5 was measured by western blots. * p < 0.05, ** p < 0.01, and *** p < 0.001.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 6 Ectopic expression of miR-578 inhibited proliferation, pro-inflammatory cytokine secretion, and ECM degradation in NP cells. A miR-578 overexpression inhibited NP cell proliferation as shown by the CCK-8 assay. B The expression of cyclin D1 was analyzed by using qRT-PCR and GAPDH was used as the internal control. C-F Overexpression of miR-578 suppressed C IL-6, D IL-8, E MMP-9, and F ADAMTS-5 expression as shown by qRT-PCR. G, H The expression of type II collagen and aggrecan was determined by qRT-PCR assay. Ectopic expression of circ_0134111 decreased G type II collagen and H aggrecan expression in NP cells. J The protein expression of MMP-9 and ADAMTS-5 was measured by western blots. * p < 0.05, ** p < 0.01, and *** p < 0.001.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FIGURE 4. I-BET151 and (+)-JQ1 affected the expression of Col2a1 , Adamts5 , and Mmp3 in chondrocytes. A-F , the expression level of chondrocyte-specific marker genes Col2a1 , Adamts5 , and Mmp3 as analyzed by quantitative RT-PCR using RNA isolated from cells treated as indicated. The values 0.2, 0.5, 1, and 5 are reported as 0.2, 0.5, 1, and 5 mu m , respectively. Values represent mean +- S.D. ( n = 3). p values were obtained from t tests with paired or unpaired samples: *, p < 0.05; **, p < 0.01. G and H , indicated protein level was assessed by Western blotting after treatment of chondrocytes with I-BET151 and (+)-JQ1. Error bars represent S.D.