14773-1-AP

antibody from Invitrogen Antibodies

Targeting: TARS1 TARS

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14773-1-AP

Provider

Invitrogen Antibodies

Product name

TARS Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: ENMFSFEVE KELFALKPMN CPGHCLMFDH RPRSWRELPL RLADFGVLHR NELSGALTGL TRVRRFQQDD AHIFCAMEQI EDEIKGCLDF LRTVYSVFGF SFKLNLSTRP EKFLGDIEVW DQAEKQLENS LNEFGEKWEL NSGDGAFYGP KIDIQIKDAI GRYHQCATIQ LDFQLPIRFN LTYVSHDGDD KKRPVIVHRA ILGSVERMIA ILTENYGGKW PFWLSPRQVM VVPVGPTCDE YAQKVRQQFH DAKFMADIDL DPGCTLNKKI RNAQLAQYNF ILVVGEKEKI SGTVNIRTRD NKVHGERTIS ETIERLQQLK EFRSKQAEEE F (394-723 aa encoded by BC000517)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.13 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A549 cells were subjected to SDS PAGE followed by western blot with 14773-1-AP (TARS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A549 cells were subjected to SDS PAGE followed by western blot with 14773-1-AP (TARS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using TARS antibody 14773-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using TARS antibody 14773-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of MCF-7 cells, using TARS antibody 14773-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 14773-1-AP ( TARS Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 14773-1-AP ( TARS Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-TARS (IP:14773-1-AP, 4ug; Detection:14773-1-AP 1:500) with A549 cells lysate 2400ug.