Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
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Validation data
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- Product number
- NB100-88130 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-88130, RRID:AB_1217831
- Product name
- Rabbit Polyclonal KNTC1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: KNTC1 Antibody [NB100-88130] - Detection of Human ROD/KNTC1 on HeLa whole cell lysate using NB100-88130. ROD/KNTC1 was also immunoprecipitated by rabbit anti-ROD/KNTC1 antibody NBN100-88131.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: KNTC1 Antibody [NB100-88130] - Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ROD/KNTC1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: KNTC1 Antibody [NB100-88130] - Detection of human KNTC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-KNTC1 antibody NB100-88130 (lot 2) used for IP at 3 ug per reaction. KNTC1 was also immunoprecipitated by a previous lot of this antibody (lot 1) and rabbit anti-KNTC1 antibody NB100-88131. For blotting immunoprecipitated KNTC1, NB100-88130 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.