AP60010PU-S

antibody from Acris Antibodies GmbH

Targeting: EMCN MUC14

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP60010PU-S

Provider

Acris Antibodies GmbH

Product name

anti Endomucin

Antibody type

Polyclonal

Antigen

Highly pure (>95%) recombinant mouse soluble Endomucin (Glu20-Ser190) derived from E. coli (Cat.-No. AR60010PU-N)

Reactivity

Mouse

Host

Rabbit

Isotype

IgG

Vial size

50 µg

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western analysis using anti-mouse Endomucin antibody Cat.-No. AP60010PU-N. Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions. Lane 1: MWM (kDa). Lane 2: rm sEndomucin (250ng).

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunofluorescence staining (green) of cryo-sections of adult mouse kidney (fixed 15 min in 4% PFA) with anti-mouse Endomucin antibody Cat.-No. AP60010PU-S (5μg/ml) and counter staining of nuclei with DAPI (blue). <i>Performed by the research group of Prof. Dr. J. Wilting and Dr. K. Buttler, University Göttingen, Germany.</i>

Flow cytometry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

FACS analysis with primary mouse endothelial cells (SnoMec).