PA5-21395

antibody from Invitrogen Antibodies

Targeting: EMCN MUC14

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-21395

Provider

Invitrogen Antibodies

Product name

Endomucin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: A431, HeLa, HepG2.

Concentration

1 mg/mL

References

Submitted references

PD-L1 on invasive fibroblasts drives fibrosis in a humanized model of idiopathic pulmonary fibrosis.

Geng Y, Liu X, Liang J, Habiel DM, Kulur V, Coelho AL, Deng N, Xie T, Wang Y, Liu N, Huang G, Kurkciyan A, Liu Z, Tang J, Hogaboam CM, Jiang D, Noble PW
JCI insight 2019 Mar 21;4(6)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Endomucin using 30 µg of A431 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with an Endomucin polyclonal antibody (Product # PA5-21395) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Endomucin Polyclonal Antibody (Product # PA5-21395). Whole cell extract (50 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with Endomucin Polyclonal Antibody (Product # PA5-21395) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Endomucin Polyclonal Antibody detects Endomucin protein at cytosol on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. Endomucin Polyclonal Antibody (Product # PA5-21395) dilution: 1:250. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.