PA5-14470

antibody from Invitrogen Antibodies

Targeting: HIPK2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-14470

Provider

Invitrogen Antibodies

Product name

HIPK2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with mouse based on sequence homology.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

2 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

CircASH2L facilitates tumor-like biologic behaviours and inflammation of fibroblast-like synoviocytes via miR-129-5p/HIPK2 axis in rheumatoid arthritis.

Li X, Qu M, Zhang J, Chen K, Ma X
Journal of orthopaedic surgery and research 2021 May 8;16(1):302

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis using a HIPK2 polyclonal antibody (Product # PA5-14470) in Ramos cell lysates (35 µg per lane).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human hepatocarcinoma tissue using a HIPK2 polyclonal antibody (Product # PA5-14470), followed by HRP-conjugated secondary antibody and DAB staining.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 5 CircASH2L indirectly regulates HIPK2 via miR-129-5p in RA-FLSs. a Binding sites of miR-129-5p on HIPK2 3''UTR. b Interaction analysis between miR-129-5p and HIPK2 using the dual-luciferase reporter assay. c - f HIPK2 expression level in synovial tissues with RA and non-RA, as well as in RA-FLSs and N-FLSs was determined. g MiR-129-5p expression analysis using qRT-PCR in RA-FLSs transfected with miR-NC or miR-129-5p. h , i HIPK2 expression analysis in miR-129-5p-increased RA-FLSs with qRT-PCR and western blot. j , k RA-FLSs were co-transfected with si-circASH2L and/or anti-miR-129-5p and HIPK2 level was examined using qRT-PCR and western blot. * P

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 6 MiR-129-5p impairs RA-FLS tumor-like biologic behaviors through HIPK2. The miR-129-5p and/or HIPK2 were co-transfected into RA-FLSs. After transfection, a western blot analysis of HIPK2 expression using western blot; b , c MTT assay of cell proliferation and Edu assay; d flow cytometry of cell apoptosis detection; e western blot analysis of apoptosis-related protein expression in cells; f flow cytometry for cell cycle analysis; g , h cell migratory and invasive abilities detection using transwell assay; i the measurement of inflammatory cytokines levels using ELISA. * P