MA5-21159

antibody from Invitrogen Antibodies

Targeting: ZNF274 ZKSCAN19, ZSCAN51

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-21159

Provider

Invitrogen Antibodies

Product name

ZNF274 Monoclonal Antibody (1D8)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

1D8

Vial size

100 µg

Concentration

0.2-1.0 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of ZNF274 expressed in transfected 293T cell line by anti-ZNF274 monoclonal antibody (Product # MA5-21159), clone 1D8. Lane 1: ZNF274 transfected lysate (74.2 KDa). Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ZNF274 over-expressed 293 cell line, cotransfected with ZNF274 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ZNF274 monoclonal antibody, clone 1D8 (Product # MA5-21159). GAPDH (36.1 kDa) used as specificity and loading control.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HeLa cells using an anti-ZNF274 monoclonal antibody (Product # MA5-21159). (antibody concentration 10 µg/mL).