Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- MA5-21159 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ZNF274 Monoclonal Antibody (1D8)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Peptide Sequence: QKIDNPESQA NSGALDTNQV LLHKIPPRKR LRKRDSQVKS MKHNSRVKIH QKSCERQKAK EGNGCRKTFS RSTKQITFIR IHKGSQVCRC SECGKIFRNP RYFSVHKKIH T
- Antibody clone number
- 1D8
- Concentration
- 0.2-1.0 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ZNF274 expressed in transfected 293T cell line by anti-ZNF274 monoclonal antibody (Product # MA5-21159), clone 1D8. Lane 1: ZNF274 transfected lysate (74.2 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ZNF274 over-expressed 293 cell line, cotransfected with ZNF274 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ZNF274 monoclonal antibody, clone 1D8 (Product # MA5-21159). GAPDH (36.1 kDa) used as specificity and loading control.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells using an anti-ZNF274 monoclonal antibody (Product # MA5-21159). (antibody concentration 10 µg/mL).