51045-1-AP

antibody from Proteintech Group

Targeting: SIK1 msk, SNF1LK

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

51045-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#51045-1-AP, RRID:AB_2187322

Product name

SIK1 antibody

Antibody type

Polyclonal

Description

KD/KO validated SIK1 antibody (Cat. #51045-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IF, IHC, WB, ELISA.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

HG-9-91-01 Attenuates Murine Experimental Colitis by Promoting Interleukin-10 Production in Colonic Macrophages Through the SIK/CRTC3 Pathway.

Fu Y, Ma G, Zhang Y, Wang W, Shi T, Zhu J, Zhang J, Huang Z, Chen J
Inflammatory bowel diseases 2021 Oct 20;27(11):1821-1831

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Role of SIK1 in the transition of acute kidney injury into chronic kidney disease.

Hu J, Qiao J, Yu Q, Liu B, Zhen J, Liu Y, Ma Q, Li Y, Wang Q, Wang C, Lv Z
Journal of translational medicine 2021 Feb 15;19(1):69

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High-salt diet downregulates TREM2 expression and blunts efferocytosis of macrophages after acute ischemic stroke.

Hu M, Lin Y, Men X, Wang S, Sun X, Zhu Q, Lu D, Liu S, Zhang B, Cai W, Lu Z
Journal of neuroinflammation 2021 Apr 12;18(1):90

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SIK1 Regulates CRTC2-Mediated Gluconeogenesis Signaling Pathway in Human and Mouse Liver Cells.

Wang C, Song D, Fu J, Wen X
Frontiers in endocrinology 2020;11:580

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Zhenqing recipe attenuates non-alcoholic fatty liver disease by regulating the SIK1/CRTC2 signaling in experimental diabetic rats.

Song D, Yin L, Wang C, Wen X
BMC complementary medicine and therapies 2020 Jan 31;20(1):27

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SIK inhibition in human myeloid cells modulates TLR and IL-1R signaling and induces an anti-inflammatory phenotype.

Lombardi MS, Gilliéron C, Dietrich D, Gabay C
Journal of leukocyte biology 2016 May;99(5):711-21

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Attenuated LKB1-SIK1 signaling promotes epithelial-mesenchymal transition and radioresistance of non-small cell lung cancer cells.

Yao YH, Cui Y, Qiu XN, Zhang LZ, Zhang W, Li H, Yu JM
Chinese journal of cancer 2016 Jun 7;35:50

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GINS complex protein Sld5 recruits SIK1 to activate MCM helicase during DNA replication.

Joshi K, Shah VJ, Maddika S
Cellular signalling 2016 Dec;28(12):1852-1862

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Role of salt-induced kinase 1 in androgen neuroprotection against cerebral ischemia.

Cheng J, Uchida M, Zhang W, Grafe MR, Herson PS, Hurn PD
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 2011 Jan;31(1):339-50

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

A549 cells were subjected to SDS PAGE followed by western blot with 51045-1-AP(SIK1 antibody) at dilution of 1:400

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of MCF-7 cells, using Sik1 antibody 51045-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human lung cancer using 51045-1-AP(SIK1 antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human lung cancer using 51045-1-AP(SIK1 antibody) at dilution of 1:100 (under 40x lens)

Sample type

tissue