51045-1-AP

antibody from Invitrogen Antibodies

Targeting: SIK1 msk, SNF1LK

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

51045-1-AP

Provider

Invitrogen Antibodies

Product name

SIK1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: ASCSGVFRP RPVSPSSLLD TAISEEARQG PGLEEEQDTQ ESLPSSTGRR HTLAEVSTRL SPLTAPCIVV SPSTTASPAE GTSSDSCLTF SASKSPAGLS GTPATQGLLG ACSPVRLASP FLGSQSATPV LQAQGGLGGA VLLPVSFQEG RRASDTSLTQ GLKAFRQQLR KTTRTKGFLG LNKIKGLARQ VCQVPASRAS RGGLSPFHAP AQSPGLHGGA AGSREGWSLL EEVLEQQRLL QLQHHPAAAP GCSQAPQPAP APFVIAPCDG PGAAPLPSTL LTSGLPLLPP PLLQTGASPV ASAAQLLDTH LHIGTGPTAL PAVPPPRLAR LAPGCEPLGL LQGDCEM (423-769 aa encoded by BC038504 )

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.13 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A549 cells were subjected to SDS PAGE followed by western blot with 51045-1-AP (SIK1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WB result of SIK1 antibody (51045-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SIK1 transfected A549 cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 51045-1-AP (SIK1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using SIK1 antibody (Product # 51045-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of MCF-7 cells, using Sik1 antibody 51045-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 51045-1-AP (Snf1lk Antibody) at dilution of 1:100 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 51045-1-AP (Snf1lk Antibody) at dilution of 1:100 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver tissue slide using 51045-1-AP (SIK1 antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver tissue slide using 51045-1-AP (SIK1 antibody) at dilution of 1:200 (under 40x lens).