AMAb91096

antibody from Atlas Antibodies

Targeting: LAMB2 LAMS, NPHS5

Western blot (Enhanced validation)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AMAb91096

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#AMAb91096, RRID:AB_2665798

Product name

Anti-LAMB2

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

DLTDVQDENFNANHALSGLERDRLALNLTLRQLDQ
HLDLLKHSNFLGAYDSIRHAHSQSAEAERRANTSA
LAVPSPVSNSASARHRTEALMDAQKEDFNSKHMAN
QRALGKLSAHTHTLSLTDINELVCGAPG


Epitope

Binds to an epitope located within the peptide sequence NANHALSGLERDRLA as determined by overlapping synthetic peptides.

Isotype

IgG

Antibody clone number

CL2976

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis in U-138MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-LAMB2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis of purified human recombinant Laminin-421, Laminin-521, Laminin-332 and Laminin-111.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human lung tissue.

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human heart shows strong membranous immunoreactivity in cardiomyocytes.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human colon shows strong positivity in basement membrane of glandular epithelium.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skeletal muscle shows strong membranous immunoreactivity in myocytes.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney shows strong positivity in renal glomeruli.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human lymph node shows absence of immunoreactivity in lymphoid cells (negative control).

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cortex shows strong immunoreactivity in endothelial cells.