AMAb91097

antibody from Atlas Antibodies

Targeting: LAMB2 LAMS, NPHS5

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

AMAb91097

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#AMAb91097, RRID:AB_2665799

Product name

Anti-LAMB2

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

DLTDVQDENFNANHALSGLERDRLALNLTLRQLDQ
HLDLLKHSNFLGAYDSIRHAHSQSAEAERRANTSA
LAVPSPVSNSASARHRTEALMDAQKEDFNSKHMAN
QRALGKLSAHTHTLSLTDINELVCGAPG


Epitope

Binds to an epitope located within the peptide sequence NANHALSGLERDRLA as determined by overlapping synthetic peptides.

Isotype

IgG

Antibody clone number

CL2979

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis of purified human recombinant Laminin-421, Laminin-521, Laminin-332 and Laminin-111.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human lung tissue.

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human heart muscle and tonsil tissues using AMAb91097 antibody. Corresponding LAMB2 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human heart shows strong membranous immunoreactivity in cardiomyocytes.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis shows strong positivity in basement membrane of seminiferous tubules.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human stomach shows moderate positivity in basement membrane of glandular epithelium.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cervix shows immunoreactivity in basement membrane of squamous epithelium.

Supportive validation

Submitted by

Atlas Antibodies (provider)

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Experimental details

Immunohistochemical staining of human skeletal muscle shows strong positivity in endomysium.

Supportive validation

Submitted by

Atlas Antibodies (provider)

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Experimental details

Immunohistochemical staining of human lymph node shows absence of immunoreactivity in lymphoid cells (negative control).

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human heart muscle shows moderate membranous positivity in cardiomyocytes.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney shows moderate membranous positivity in basement membrane of cells in glomeruli.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human endometrium shows moderate positivity in basement membrane of glandular cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human tonsil shows no positivity in lymphoid cells as expected.