MAB1334-100

antibody from R&D Systems

Targeting: IGFBP7 FSTL2, IGFBP-7, MAC25, PSF

Western blot (Data presented on provider website)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB1334-100

Provider

R&D Systems

Product name

Human IGFBP-rp1/IGFBP-7 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human IGFBP-rp1/IGFBP-7 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human IGFBP-1, -2, -3, -4, -5, or -6 is observed.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

AAA16187


Isotype

IgG

Antibody clone number

192520

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

IGFBP7 Induces Differentiation and Loss of Survival of Human Acute Myeloid Leukemia Stem Cells without Affecting Normal Hematopoiesis.

Verhagen HJMP, van Gils N, Martiañez T, van Rhenen A, Rutten A, Denkers F, de Leeuw DC, Smit MA, Tsui ML, de Vos Klootwijk LLE, Menezes RX, Çil M, Roemer MGM, Vermue E, Heukelom S, Zweegman S, Janssen JJWM, Ossenkoppele GJ, Schuurhuis GJ, Smit L
Cell reports 2018 Dec 11;25(11):3021-3035.e5

---

Biomarker discovery from pancreatic cancer secretome using a differential proteomic approach.

Grønborg M, Kristiansen TZ, Iwahori A, Chang R, Reddy R, Sato N, Molina H, Jensen ON, Hruban RH, Goggins MG, Maitra A, Pandey A
Molecular & cellular proteomics : MCP 2006 Jan;5(1):157-71

ELISA

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Human IGFBP-rp1/IGFBP-7 ELISA Standard Curve. Recombinant Human IGFBP-rp1/IGFBP-7 protein was serially diluted 2-fold and captured by Mouse Anti-Human IGFBP-rp1/IGFBP-7 Monoclonal Antibody (Catalog # MAB13342) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human IGFBP-rp1/IGFBP-7 Monoclonal Antibody (Catalog # MAB1334) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).