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- Antibody Data
- Antigen structure
- References [1]
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- Western blot [2]
- ELISA [1]
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- Product number
- 500-P234-100UG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IGFBP7 Polyclonal Antibody, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: SSSDTCGPCE PASCPPLPPL GCLLGETRDA CGCCPMCARG EGEPCGGGGA GRGYCAPGME CVKSRKRRKG KAGAAAGGPG VSGVCVCKSR YPVCGSDGTT YPSGCQLRAA SQRAESRGEK AITQVSKGTC EQGPSIVTPP KDIWNVTGAQ VYLSCEVIGI PTPVLIWNKV KRGHYGVQRT ELLPGDRDNL AIQTRGGPEK HEVTGWVLVS PLSKEDAGEY ECHASNSQGQ ASASAKITVV DALHEIPVKK GEGAEL. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IGF-BP7. Anti-Human IGF-BP7-specific antibody was purified by affinity chromatography employing an immobilized Human IGF-BP7 matrix. Sandwich ELISA: To detect Human IGF-BP7 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IGF-BP7 (500-P234Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IGF-BP7. Western Blot: To detect Human IGF-BP7 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IGF-BP7 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100 µg
- Concentration
- 0.1-1.0 mg/mL
- Storage
- -20°C
Submitted references Lipid Supplement in the Cultural Condition Facilitates the Porcine iPSC Derivation through cAMP/PKA/CREB Signal Pathway.
Zhang W, Wang H, Zhang S, Zhong L, Wang Y, Pei Y, Han J, Cao S
International journal of molecular sciences 2018 Feb 8;19(2)
International journal of molecular sciences 2018 Feb 8;19(2)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect Human IGF-BP7 by Western Blot analysis IGFBP7 Polyclonal Antibody (Product # 500-P234-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IGF-BP7 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect Human IGF-BP7 by Western Blot analysis IGFBP7 Polyclonal Antibody (Product # 500-P234-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IGF-BP7 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA: To detect Human IGF-BP7 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of IGFBP7 Polyclonal Antibody (Product # 500-P234-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech IGFBP7 Polyclonal Antibody, Biotin (Product # 500-P234BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IGF-BP7.
