CABT-B9283

antibody from Creative Diagnostics

Targeting: UNC13B hmunc13, Munc13-2, UNC13, Unc13h2

Western blot (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Antibody Data

Product number

CABT-B9283

Provider

Creative Diagnostics

Product name

Mouse anti-Rat panMunc13 monoclonal antibody, clone 43

Antibody type

Monoclonal

Description

Release of neurotransmitters at the synaptic junction is a primary mechanism of neuronal communication and is regulated by the synaptic vesicle (SV) cycle. Several components of the SV cycle were initially characterized in C. elegans. One of those, unc-13, is involved in normal presynaptic function. Mammalian homologues of unc-13 are Munc13-1, 13-2, and 13-3. These neuron-specific proteins lack N-terminal homology, but are highly conserved in their C-terminal regions. Here they contain a phorbal ester binding C1-domain and two C2-domains with homology to the PKC Ca2+/phospholipid-binding domain. The most abundant isoform, Munc 13-1, is a presynaptic receptor with high affinities for phorbol ester and DAG. In response to phorbol ester, Munc13-1 associates with the plasma membrane and acts as a stimulation dependent enhancer of neurotransmitter release. In addition, Munc13-1 interacts with components of the SV cycle (syntaxin, SNAP25, synaptobrevin, Doc2) and with a brain specific isoform of ÉÇ-spectrin, a protein that interacts with the actin cytoskeleton. Thus, it is thought that Munc13 proteins function in signaling pathways that regulate the neuronal exocytic machinery.

Reactivity

Mouse, Rat

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

43

Vial size

50ug, 150ug

Concentration

250 Ã×g/ml

Storage

Store undiluted at -20°C.