HPA056230

antibody from Atlas Antibodies

Targeting: AKAP12 AKAP250, SSeCKS

Antibody data

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Validation data

Product number: HPA056230 - Provider product page
Provider: Atlas Antibodies
Proper citation: Atlas Antibodies Cat#HPA056230, RRID:AB_2683073
Product name: Anti-AKAP12
Antibody type: Polyclonal
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Antigen sequence: SKDMSEASEKTMTVEVEGSTVNDQQLEEVVLPSEE EGGGAGTKSVPEDDGHALLAERIEKSLVEPKEDEK GDDVDDPENQNSALADTDASGGLTKESPDTNGPKQ KEKEDAQEVELQEGKVHSESDKAITPQAQEELQKQ ERESAKS
Isotype: IgG
Vial size: 100 µl
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

AKAP12 protein structure - HPA056230 shown in red.

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
Main image
Experimental details: Immunohistochemistry analysis in human testis and skeletal muscle tissues using Anti-AKAP12 antibody. Corresponding AKAP12 RNA-seq data are presented for the same tissues.
Sample type: HUMAN
Orthogonal method: Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Independent antibody validation
Main image
Experimental details: Immunohistochemical staining of human kidney, liver, skeletal muscle and testis using Anti-AKAP12 antibody HPA056230 (A) shows similar protein distribution across tissues to independent antibody HPA006344 (B).
Antibody #2 product nr: HPA006344
Antibody provider: Atlas Antibodies

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human testis shows high expression.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human skeletal muscle shows low expression as expected.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human liver using Anti-AKAP12 antibody HPA056230.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human kidney using Anti-AKAP12 antibody HPA056230.
Sample type: HUMAN