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Western blot
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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- AP52190PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP52190PU-N, RRID:AB_11146650
- Product name
- anti Interleukin-18 / IL18 (Center)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide - KLH conjugated - corresponding to the central region (between 44-73aa) of human Interleukin-18 / IL18.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 0.25 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of IL18 (arrow) using Interleukin-18 / IL18 antibody Cat.-No. AP52190PU-N; 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the IL18 gene (Lane 2).
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis in MDA-MB468 cell line lysates (35ug/lane) using Interleukin-18 / IL18 antibody Cat.-No. AP52190PU-N. This demonstrates the IL18 antibody detected the IL18 protein (arrow).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in human hepatocarcinoma tissue (Formalin-fixed, Paraffin-embedded) using Interleukin-18 / IL18 antibody Cat.-No. AP52190PU-N, followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IL18 antibody for IHC; Clinical relevance has not been evaluated.
