A301-145A
antibody from Invitrogen Antibodies
Targeting: NCOR1
hCIT529I10, hN-CoR, KIAA1047, MGC104216, N-CoR, PPP1R109, TRAC1
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Chromatin Immunoprecipitation [1]
- Other assay [1]
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Validation data
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- Product number
- A301-145A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NCoR Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.20 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse NCoR by western blot. Samples:Whole cell lysate (50 µg) from HEK293T, K-562, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NCoR antibody (Product # A301-145A; lot A301-145A-3) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human NCoR by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NCoR antibody A301-145A (lot A301-145A-2) used for IP at 6 µg per reaction. NCoR was also immunoprecipitated by rabbit anti-NCoR antibody A301-146A. For blotting immunoprecipitated NCoR, A301-145A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse NCoR by western blot. Samples: Whole cell lysate (5, 15 and 50 µg) from HeLa and (50 µg) from mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NCoR antibody A301-145A (lot A301-145A-2) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Localization of NCoR Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-NCoR antibody (Product # A301-145A) and analyzed by DNA sequencing. The figure illustrates the peak distribution of NCoR binding within a 650 Kb region of the human X chromosome as detected using anti-NCoR antibody (Product # A301-145A). ChIP-seq validation performed by Diogenode, Denville, NJ.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human NCoR by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NCoR antibody (Product # A301-145A; lot A301-145A-3) used for IP at 6 µg per reaction. NCoR was also immunoprecipitated by a previous lot of this antibody (lot A301-145A-2) and rabbit anti-NCoR antibody (Product # A300-146A). For blotting immunoprecipitated NCoR (Product # A301-145A) was used at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.