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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- UM500083 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#UM500083, RRID:AB_2629095
- Product name
- ALX4 mouse monoclonal antibody,clone UMAB118
- Antibody type
- Monoclonal
- Description
- ALX4 mouse monoclonal antibody,clone UMAB118
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- ALX4
- Isotype
- IgG
- Antibody clone number
- UMAB118
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot of cell lysates (35ug) from 8 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: MCF7, 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12). Diluation: 1:500.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of FFPE human breast tissue section using anti-ALX4 mouse monoclonal antibody UM500083, clone UMAB118 at 1:200. (Heat-induced epitope retrieval by Tris-EDTA, pH9.0; Polink1 Broad HRP for 15 min; DAB chromogen for 5 min & DAB enhancer for 30 sec).
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-ALX4 mouse monoclonal antibody (UM500083). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.
- Validation comment
- 10K-CHIP
