Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [9]
- Immunohistochemistry [4]
- Other assay [1]
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Validation data
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- Product number
- 16951-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KLHL3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MEGESVKLS SQTLIQAGDD EKNQRTITVN PAHMGKAFKV MNELRSKQLL CDVMIVAEDV EIEAHRVVLA ACSPYFCAMF TGDMSESKAK KIEIKDVDGQ TLSKLIDYIY TAEIEVTEEN VQVLLPAASL LQLMDVRQNC CDFLQSQLHP TNCLGIRAFA DVHTCTDLLQ QANAYAEQHF PEVMLGEEFL SLSLDQVCSL ISSDKLTVSS EEKVFEAVIS WINYEKETRL EHMAKLMEHV RLPLLPRDYL VQTVEEEALI KNNNTCKDFL IEAMKYHLLP LDQRLLIKNP RTKPRTPVSL PK (1-301 aa encoded by BC034035)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.13 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse liver tissue were subjected to SDS PAGE followed by western blot with 16951-1-AP (KLHL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 16951-1-AP (KLHL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 16951-1-AP (KLHL3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 16951-1-AP (KLHL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Rat liver tissue were subjected to SDS PAGE followed by western blot with 16951-1-AP (KLHL3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human liver tissue were subjected to SDS PAGE followed by western blot with 16951-1-AP (KLHL3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse thymus tissue were subjected to SDS PAGE followed by western blot with 16951-1-AP (KLHL3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HL-60 cells were subjected to SDS PAGE followed by western blot with 16951-1-AP (KLHL3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 16951-1-AP (KLHL3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human tonsillitis slide using 16951-1-AP (KLHL3 Antibody) at dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human tonsillitis slide using 16951-1-AP (KLHL3 Antibody) at dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver using 16951-1-AP (KLHL3 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver using 16951-1-AP (KLHL3 antibody) at dilution of 1:100 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-KLHL3 (IP:16951-1-AP, 4ug; Detection:16951-1-AP 1:700) with HepG2 cells lysate 2800ug.