AM2157b

antibody from Abgent

Targeting: SDC1 CD138, SDC, SYND1, syndecan

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM2157b

Provider

Abgent

Product name

CD138 Antibody

Antibody type

Monoclonal

Antigen

Other

Description

Mouse Monoclonal Antibody (Mab)

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

400 µl

Concentration

0.5 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

CD138 Antibody (Cat. #AM2157a) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the CD138 antibody detected the CD138 protein (arrow).

Primary Ab dilution

1:100~500

Immunocytochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Confocal immunofluorescent analysis of CD138 antibody (Cat#AM2157a) with RPMI8266 cell (above) compared with Jurkat as negative cell line (below).followed by DyLight 488-conjugated goat anti-mouse lgG (H+L) Secondary Antibody (green).DAPI was used to stain the cell nucleus (blue).

Primary Ab dilution

1:10~50

Flow cytometry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

CD138 Antibody flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).Alexa Fluor 488-conjugated donkey anti-mouse lgG secondary antibodies were used for the analysis

Primary Ab dilution

1:10~50