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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- F40400 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Syndecan-1 Antibody
- Antibody type
- Monoclonal
- Antigen
- This Syndecan-1 antibody was produced from mice immunized with a KLH conjugated synthetic peptide selected from the human protein.
- Description
- Purified antibody
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 480CT5.4.3
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the Syndecan-1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Syndecan-1 antibody western blot analysis in T47D lysate. Predicted molecular weight: 20-95 kDa depending on glycosylation level.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of Syndecan-1 antibody with RPMI8266 cells (above) compared with Jurkat as negative cell line (below), followed by secondary Ab (green). DAPI was used to stain the cell nucleus (blue).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Syndecan-1 antibody flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control (left histogram). Alexa Fluor 488-conjugated donkey anti-mouse lgG secondary Ab was used for the analysis
