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LearnH00005495-M01
antibody from Novus Biologicals
Targeting: PPM1B
PP2CB, PP2CBETA, PPC2BETAX
Western blot ELISA Immunocytochemistry Immunoprecipitation
Blocking/Neutralizing Proximity ligation assayAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- H00005495-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005495-M01, RRID:AB_547241
- Product name
- Mouse Monoclonal PP2C beta/PPM1B Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. PPM1B - protein phosphatase 1B (formerly 2C), magnesium-dependent, beta isoform
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references 14-3-3 epsilon dynamically interacts with key components of mitogen-activated protein kinase signal module for selective modulation of the TNF-alpha-induced time course-dependent NF-kappaB activity.
Zuo S, Xue Y, Tang S, Yao J, Du R, Yang P, Chen X
Journal of proteome research 2010 Jul 2;9(7):3465-78
Journal of proteome research 2010 Jul 2;9(7):3465-78
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PP2C beta/PPM1B Antibody (1A3-2A4) [H00005495-M01] - Analysis of PPM1B over-expressed 293 cell line, cotransfected with PPM1B Validated Chimera RNAi ( Cat # H00005495-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PPM1B monoclonal antibody (M01), clone 1A3-2A4 (Cat # H00005495-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PP2C beta/PPM1B Antibody (1A3-2A4) [H00005495-M01] - Analysis of PPM1B expression in transfected 293T cell line by PPM1B monoclonal antibody (M01), clone 1A3-2A4.Lane 1: PPM1B transfected lysate(52.643 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: PP2C beta/PPM1B Antibody (1A3-2A4) [H00005495-M01] - Detection limit for recombinant GST tagged PPM1B is 0.3 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: PP2C beta/PPM1B Antibody (1A3-2A4) [H00005495-M01] - Analysis of monoclonal antibody to PPM1B on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: PP2C beta/PPM1B Antibody (1A3-2A4) [H00005495-M01] - Analysis of PPM1B transfected lysate using anti-PPM1B monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PPM1B monoclonal antibody.
