AF1219

antibody from R&D Systems

Targeting: ITGAV CD51, MSK8, VNRA, VTNR

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

AF1219

Provider

R&D Systems

Product name

Human/Mouse/Rat Integrin alpha V/CD51 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human Integrin alpha V in direct ELISAs and Western blots. Detects human, mouse and rat Integrin alpha V in Simple Western application. In Western blots, less than 1% cross-reactivity with recombinant human Integrin alpha 5 and recombinant mouse Integrin E is observed.

Reactivity

Human, Mouse, Rat

Host

Goat

Conjugate

Unconjugated

Antigen sequence

P06756


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Characterization of Human and Murine T-Cell Immunoglobulin Mucin Domain 4 (TIM-4) IgV Domain Residues Critical for Ebola Virus Entry.

Rhein BA, Brouillette RB, Schaack GA, Chiorini JA, Maury W
Journal of virology 2016 Jul 1;90(13):6097-6111

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Quantity and accessibility for specific targeting of receptors in tumours.

Hussain S, Rodriguez-Fernandez M, Braun GB, Doyle FJ 3rd, Ruoslahti E
Scientific reports 2014 Jun 10;4:5232

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Binding of extracellular maspin to beta1 integrins inhibits vascular smooth muscle cell migration.

Bass R, Wagstaff L, Ravenhill L, Ellis V
The Journal of biological chemistry 2009 Oct 2;284(40):27712-20

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human, Mouse, and Rat Integrin alpha V/CD51 by Simple Western^TM. Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, A549 human lung carcinoma cell line, C6 rat glioma cell line, and NIH-3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Integrin alpha V/CD51 at approximately 149-161 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Mouse/Rat Integrin alpha V/CD51 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1219) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Integrin alpha V/CD51 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells, A549 human lung carcinoma cell line, and HT1080 human fibrosarcoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Integrin alpha V/CD51 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1219) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Integrin alpha V/CD51 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Integrin alpha V/CD51 in Rat Mesenchymal Stem Cells. Integrin alpha V/CD51 was detected in immersion fixed undifferentiated rat mesenchymal stem cells using Goat Anti-Human/Mouse/Rat Integrin alpha V/CD51 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1219) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.