F54435

antibody from NSJ Bioreagents

Targeting: COMP EDM1, EPD1, MED, PSACH, THBS5

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F54435

Provider

NSJ Bioreagents

Product name

COMP Antibody / TSP5

Antibody type

Polyclonal

Description

Antigen affinity purified

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 µl, 0.4 µl

Storage

Aliquot the TSP5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human skeletal muscle, 2) mouse ATDC5, 3) HUVEC, 4) human Jurkat, 5) human MG-63 and 6) human U-2 OS lysate with TSP5 antibody. Expected molecular weight: 83-100 kDa depending on glycosylation level.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of rat skeletal muscle lysate with TSP5 antibody. Expected molecular weight: 83-100 kDa depending on glycosylation level.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human prostate carcinoma tissue with TSP5 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of fixed and permeabilized human HepG2 cells with TSP5 antibody; Blue=isotype control, Green= TSP5 antibody.