MA5-23859

antibody from Invitrogen Antibodies

Targeting: TNFSF8 CD153, CD30LG

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-23859

Provider

Invitrogen Antibodies

Product name

CD153 Monoclonal Antibody (116614)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

In direct ELISAs, no cross-reactivity with recombinant human (rh) CD27 Ligand, recombinant mouse CD30 Ligand, or rhCD40 Ligand is observed.

Antibody clone number

116614

Concentration

0.5 mg/mL

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of CD153 in Human peripheral blood mononuclear cells (PBMCs) treated with 50 ng/mL PMA and 200 ng/mL Calcium Ionomycin overnight. Samples were incubated in either (A) CD153 monoclonal antibody (Product # MA5-23859) or (B) Mouse IgG2B Isotype Control followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Neutralization of CD153 in HDLM human Hodgkin’s lymphoma cell line. Samples were incubated in CD153 monoclonal antibody (Product # MA5-23859) using a dilution of 1 µg/mL. In the presence of a cross-linking antibody, Mouse polyHistidine Monoclonal Antibody (10 µg/mL), Recombinant Human CD30 Ligand/TNFSF8 stimulates IL‚6 secretion in the HDLM human Hodgkin’s lymphoma cell line in a dose-dependent manner (orange line), as measured by the Human IL‚6 Quantikine ELISA Kit. Under these conditions, IL‚6 secretion elicited by Recombinant Human CD30 Ligand/TNFSF8 is neutralized (green line) by increasing concentrations of Mouse Anti-Human CD30 Ligand/TNFSF8 Monoclonal Antibody. The ND50 is typically 1-4 µg/mL.