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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- ARG20528 - Provider product page
- Provider
- Arigo
- Product name
- anti-Cofilin phospho (Ser3) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH-conjugated phosphospecific peptide around Ser3 in human Cofilin 1 protein. This sequence has 100% homology with similar regions of rat and mouse Cofilin 1, and has two amino acid differences from human Cofilin 2.
- Description
- Affinity purification with immunogen.
- Reactivity
- Human, Mouse, Rat, Chicken/Avian
- Host
- Rabbit
- Vial size
- 100 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to 1-2 weeks. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: Jurkat cells 1) untreated or 2,3) treated with lambda phosphatase, 3) the phosphatase was inhibited with phospho-Cofilin 1 (Ser3) peptide. The blots were stained with ARG20528 anti-cofilin phospho (Ser3) antibody.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunocytochemistry: chick dorsal root ganglion neurons stained with anti-Cofilin antibody (upper) and ARG20528 anti-cofilin phospho (Ser3) antibody (lower). Images provided by Dr. Diane Snow, Department of Anatomy & Neurobiology, University of Kentucky.
